Elucidating mechanism cellular uptake removal protein
The length of the needle was cut to make sure the cells would be hanged approximately 1.2 mm above the bottom of the well, thus minimizing the difference in liquid height above the cells between the two configurations.By using the same batch of coverslips for all experiments, we could reduce the difference in cell morphology and uptake activity caused by dissimilar surfaces.Here we use upright and inverted cell culture configurations to show that cellular uptake of gold nanoparticles depends on the sedimentation and diffusion velocities of the nanoparticles and is independent of size, shape, density, surface coating and initial concentration of the nanoparticles.Generally more nanoparticles are taken up in the upright configuration than the inverted one and nanoparticles that sediment faster showed greater differences in uptake between the two configurations.Anti-ulcer and in vivo antioxidant activity of methanolic extracts of Mallotus roxburghianus as conventional preparations and as nano-encapsulated formulation in ethanol-induced gastric ulcer in rats SAM HIGGINBOTTOM INSTITUTE OF AGRICULTURE, TECHNOLOGY & SCIENCES., C/O Dr. 35-6-770/202, Suryabhanu Residency, TV tower colony, KU 'X' Road, Hanamkonda, Warangal, TS 506001, Bhubaneswar, Orissa- 751004 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Naini Allahabad, Dept.
This way, we could easily position the cells in an inverted configuration right before an uptake experiment by inserting the syringe needle into the rubber block.
After the nanoparticles had been incubated with the cells, the LSPR peaks were observed to drop in intensity.
Notably, the drop was greater in the upright configuration than in the inverted configuration for the same sample of nanoparticles.
We anticipate that the disparity in cellular uptake between the two configurations will be negligible when diffusion prevails over sedimentation, and vice versa.
In this study, we examined six different types of gold nanoparticles: nanospheres of three sizes, nanocages of two edge lengths, and nanorods (Supplementary Method S1).